Salta al contenuto principale
  • Partner approvati
  • A proposito di ELGA
    • A proposito di ELGA
    • Carriere
    • Eventi
  • Supporto
    • Laboratory Planning
    • Registra un Prodotto
    • Register Your Product (USA & Canada Only)
  • Contatti
  • U.S.A.
  • U.K.
  • Deutschland
  • España
  • France
  • Brasil
  • 日本
  • 中国
Home ELGA LabWater
  • Prodotti
    • PURELAB
    • CENTRA
    • MEDICA
    • BIOPURE
    • ELGA Gamma Completa di Prodotti
  • Applicazioni
    • Analisi microbiologica
    • Biochimica clinica
    • Colture cellulari
    • Cromatografia liquida
    • Elettrochimica
    • Gascromatografia
    • Genetica
    • Immunochimica
    • Requisiti dell'acqua per laboratori generici
    • Spettrofotometria
    • Spettrometria di massa
    • Spettroscopia atomica
  • Tecnologie
    • Activated Carbon
    • Electrodeionization (EDI)
    • Filtration
    • Ion Exchange
    • PureSure
    • Reverse Osmosis
    • Ultraviolet
  • Impurità nell'acqua
    • Dissolved Gases
    • Inorganic Compounds
    • Microorganisms & Bacteria
    • Organic Compounds
    • Particulates
  • Knowledge
    • Blog
    • Casi di studio
    • Acqua ultrapura
    • Guide e libri bianchi
  • Prodotti
    • PURELAB
    • CENTRA
    • MEDICA
      • Hubgrade
      • MEDICA BIOX
    • BIOPURE
    • ELGA Gamma Completa di Prodotti
      • PURELAB® Classic
  • Applicazioni
    • Analisi microbiologica
    • Biochimica clinica
    • Colture cellulari
    • Cromatografia liquida
      • Hochleistungsflüssigkeitschromatographie (HPLC)
    • Elettrochimica
    • Gascromatografia
    • Genetica
    • Immunochimica
    • Requisiti dell'acqua per laboratori generici
    • Spettrofotometria
    • Spettrometria di massa
    • Spettroscopia atomica
  • Tecnologie
    • Activated Carbon
    • Electrodeionization (EDI)
    • Filtration
    • Ion Exchange
    • PureSure
    • Reverse Osmosis
    • Ultraviolet
  • Impurità nell'acqua
    • Dissolved Gases
    • Inorganic Compounds
    • Microorganisms & Bacteria
    • Organic Compounds
    • Particulates
  • Knowledge
    • Blog
      • Analytical Chemistry
      • Clinical & Pharma
      • Cool Science
      • Environment and sustainability
      • Life in the lab
      • Purelab product design
      • Science of the future
      • Water Purity
      • Water in the lab
    • Casi di studio
      • Abbott Diagnostics chooses ELGA MEDICA systems in South Asia
      • Beam Me Up, Scotty: PURELAB® Option Q Delivers Essential Ultra Pure Water
      • Argenta chooses DKSH New Zealand to deliver Ultrapure water in Animal Pharma
      • Clean Water for a Clean Future
      • LS Scientific & ELGA deliver UltraPure water to the NAFDAC Laboratory
      • Lifebrain Group chooses ELGA as water partner for new modern 24/7 PCR-COVID-19 laboratory in Vienna, Austria.
      • Critital Tests Benefit from PURELAB® Option Reliable Pure Water
      • DASA: The biggest medical diagnostics company in Brazil
      • ELGA helps immuneserology labs maximize uptime
      • Fondazione Telethon Continues to Choose ELGA Labwater as a Trusted Partner
      • Importance of ultrapure Type 1+ water for the development of generic medicines
      • MEDICA® Pro selected for Siemens ADVIA® analyzers at City General Hospital
      • Optimale Wasserqualität für mikrobiologische Forschung und Lehre
      • PURELAB® Option R Guarantees Pure Water for Leading Microfluidics Technology
      • PURELAB® Pulse Delivers Reliable Water Quality and Quantity for a Wide Range of Applications
      • PURELAB® flex: an ideal training system for today's research method
      • Powering Cutting-Edge Gene Research
      • Sichere Reinstwasserversorgung für präzise Produkttests
      • Zentrale Reinstwasser-Aufbereitung für Analyser
      • ELGA LabWater and Beckman Coulter Join Forces
      • Applied New Technologies Department Improves ICP, IC & HPLC Sample Turnaround Times with PURELAB®
      • Unlocking the secrets of the Antarctic with the aid of ultrapure water
      • Advancing Genetic Technologies
      • Cross Infection Control: Pure and Simple
      • Researching effective new ways to prevent cardiovascular disease at the University of Columbia
    • Acqua ultrapura
    • Guide e libri bianchi
      • Purezza dell'acqua nell'HPCL
      • L'HPLC in farmacologia
      • Ridurre i rischi clinici
      • Sostenibilità
  • U.S.A.
  • U.K.
  • Deutschland
  • España
  • France
  • Brasil
  • 日本
  • 中国
  • Normativa sulla privacy
  • Termini e condizioni
  • Dichiarazioni di conformità legale globale
  • Brevetti
  • Impressum
  • Home
  • Measuring indigestible sugars in breast milk
Life Science Results

Measuring indigestible sugars in breast milk

22 Set 2022
- by Dr Alison Halliday

Measuring indigestible sugars in breast milk

Researchers develop an efficient, sensitive method to measure the levels of key oligosaccharides in human milk – helping to facilitate future research into their potential health benefits for babies.

Human milk oligosaccharides (HMOs) are the third most abundant solid component of human milk after lactose and lipids. A structurally diverse group of complex indigestible sugars, the concentrations and compositions of these compounds in human breast milk are reported to be closely related to various aspects of infant health.


HMOs are not broken down for energy but instead stay inside the gut where they help promote the growth of beneficial bacteria, such as Bifidobacterium, as well as blocking pathogenic organisms from attaching to the intestinal wall and causing infection.

Understanding the natural occurrence and biological role of HMOs could lead to improved infant milk formulations to extend certain health advantages that might previously be exclusive to breastfed babies.

Major classes

Human breast milk contains three major types of HMO based on their structural and chemical properties: fucosylated HMOs (35-50%), sialylated HMOs (12-14%) and non-fucosylated neutral HMOs (42%-55%).

The fucosylated HMOs are thought to help prevent infant diarrhoea while the sialylated HMOs have various suggested beneficial effects including helping to promote healthy brain development. Lacto-N-tetraose (LNT) and Lacto-N-neotetraose (LNnT), which are prominent non-fucosylated neutral HMOs, are known substrates for Bifidobacterium growth – supporting their role as prebiotics.

In a new study, published in Applied Food Research, researchers set out a sensitive, selective, robust and fast analytical method to simultaneously separate and quantify key pairs of HMOs in human milk.1

Excellent performance

The researchers applied ultra-high performance liquid chromatography (UHPLC) coupled with tandem mass spectrometry (LC-MS/MS) to resolve two pairs of neutral HMOs (2’-fucosyl lactose (2’FL) and 3-fucosyl lactose (3-FL) - and lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNnT)) as well as a pair of acidic HMOs (3’-sialyl lactose (3’-SL) and 6’-sialyl lactose (6’-SL)) either by chromatographic separation or unique fragment ions (MRMs).

The team validated the method by measuring the concentration of these six HMOs in eleven real human milk samples, which (except for 2’-FL in one sample) were determined to be in the range of 2.83 ± 0.20 g/L.

The researchers used ultrapure water generated from an ELGA PURELAB® laboratory water purification system for the sample preparation stage, minimising the risk of adding potential contaminants that could affect their results.

Simple, fast and robust

This new analytical method successfully resolves similar HMO isomers either by chromatographic separation (2’-FL vs 3-FL, 3’-SL vs 6’-SL) or unique fragment ions (LNT vs LNnT). These six compounds were selected due to their high abundance in human milk – and they also represent the three main classes of HMOs.

The protocol offers several advantages including simple and fast sample preparation, short total run time, simultaneous determination of neutral and acidic HMO species – and the ability to separate and quantify structurally similar isomers.

Ultimately, this method could provide a scientific basis for the development of relevant infant foods to meet the nutritional needs of children. With the continued advancement of HMO fortification in infant formula, it also has the potential to be used as a reference for food product quality control.

Why choose ELGA LabWater

ELGA LabWater has been a trusted name in pure and ultrapure water since 1937. Our dedication to ultrapure and pure water is a guarantee that we will continue to provide the best solutions with the best service.

 

Reference:

  1. Tan, J. et al. Simultaneous determination of neutral and acidic human milk oligosaccharides (HMOs) by liquid chromatography with tandem mass spectrometry (LC-MS/MS). App Food Res 2022;2(2):100153 doi: https://doi.org/10.1016/j.afres.2022.100153


 

Dr Alison Halliday

After completing an undergraduate degree in Biochemistry & Genetics at Sheffield University, Alison was awarded a PhD in Human Molecular Genetics at the University of Newcastle. She carried out five years as a Senior Postdoctoral Research Fellow at UCL, investigating the genes involved in childhood obesity syndrome. Moving into science communications, she spent ten years at Cancer Research UK engaging the public about the charity’s work. She now specialises in writing about research across the life sciences, medicine and health.

 

 

 

  • Enquiry
  • Richiedi un Preventivo
  • Prenota una Demo
  • Trova un Partner Approvato

Enquiry

Please check this to confirm that you have read our Terms of Service and Privacy Policy.

Richiedi un Preventivo

Please check this to confirm that you have read our Terms of Service and Privacy Policy.

Prenota una Demo

Please check this to confirm that you have read our Terms of Service and Privacy Policy.

Call us

Can't find what you are looking for?

Support Number
+44 (0)20 3567 7300
United Kingdom Sales
+44 (0)1628 879 704
United States of America Sales
+1 877-315-3542
France Sales
+33 1 40 83 65 00
China Sales
+86 400-616-8882

 

ELGA LabWater US Headquarters

Lane End Business Park
Lane End, High Wycombe
HP14 3BY
United Kingdom
T: +44 (0) 203 567 7300
F: +44 (0) 203 567 7205

ELGA LabWater Sede Centrale

Lane End Business Park
Lane End, High Wycombe
HP14 3BY
United Kingdom
T: +44 (0) 203 567 7300
F: +44 (0) 203 567 7205

Casi di Studio

  • Abbott Diagnostics
  • DASA Diagnostica Medica
  • NeoDIN Istituto Medico
  • North Staffordshire NHS Trust
  • Scuola Professionale Olsberg

Risorse

  • Informazioni sull'acqua ultrapura
  • Guide e libri bianchi
  • Tecnologie per la purificazione dell'acqua
  • Applicazioni di laboratorio
  • Impurità nell'acqua

Blogs

  • Latest Blog
  • Water Purity - Different Types of Pure Water
  • What is Clinical Laboratory Reagent Water (CLSI)?
  • What is Total Organic Carbon (TOC)?
  • Lingua
    • Deutsch
    • English
    • Español
    • Français
    • Italiano
    • Português
    • 日本語
    • 中文
  • Altri Siti Veolia
    • Veolia
    • Veolia Foundation
    • Veolia Water Technologies

© VWS (UK) Ltd. che opera come ELGA LabWater. 2023 - Tutti i diritti riservati.
ELGA è il marchio mondiale dell'acqua di laboratorio di Veolia.

  • Normativa sulla privacy
  • Termini e condizioni
  • Dichiarazioni di conformità legale globale
  • Brevetti
  • Impressum
Elga Veolia
TOP

© 2017 ELGA Veolia